Three detections for Bird Flu

27 November 2008

The direct detection of the existence of the virus to the bird flu sufferer was the inspection that really was hoped for. The existence of the virus that was detected this was strong proof the existence of the infection that just happened, so as to be able to be taken by the action immediately, was good for penangan the sufferer and the epidemiological handling. There were several inspection techniques for authentication of the existence of the virus to the sufferer, that is by detecting antigens (the part) the virus, culture and PCR. Deteksi of antigens were the inspection that was based on the reaction between virus parts and antibodies towards this part. This inspection was the inspection imunologis, that was the same as the antibodies inspection, but technically was inside out. So we have had antibodies for the bird flu virus, afterwards direaksikan with the specimen (for example the throat caress) the sufferer.

This inspection was easy and fast, but more insensitive, so as often the false negative happened (had the virus, but was not detected because the amount too few to put forward positive results). The detection of the virus directly also could be used by means of burying the specimen that it was suspected contained this virus in the laboratory. This inspection was very sensitive (because of the virus dikembang bred in the laboratory, so as to become many), but needed time at least three days, but also needed very safe laboratory facilities (BSL the III level). The BSL III laboratory with the level of the security was very high, was good for protected the worker and the laboratory environment and the community. Indonesia still in the process of the development of this laboratory kind, because of the procurement cost and operational him that was high. So this culture technique was not ideal for the handling of cases of the emergency with the illness trip that was fast like bird flu.

PCR was the abbreviation from Polymerase Chain Reaction, was the inspection technique that was based on the increase in the nucleic acid from the virus. To PCR this the specimen from the sufferer at once was done by the extraction of the nucleic acid (his cell was destroyed) so as for the further work only with the sample that took the form of the nucleic acid then. This inspection was fast (around 4 hours), sensitive (because in this reaction the virus nucleic acid will be doubled to millions of times), and safe (only worked with the nucleic acid that not infeksius again) so as to be able to be done to the laboratory with the level of the BSL security the level Ii, although to check the bird flu specimen, his method of operation the worker must apply principles worked like to the BSL III laboratory.

Considering various surpluses from this PCR technique, and the width of the Indonesian territory, the government tried to place this implement in various health services in the area. Also made the network of the Regional Avian Influenza Laboratory in several places (one of them in the Mikrobiologi FK UNDIP Part), so as to be hoped by the certain diagnosis bird flu could be known quickly, must not be sent to Jakarta or Hong Kong. About the diagnosis strategy better be considered on the trip of the virus infection H5N1 this. To he explained could be seen in the picture 2, where being depicted that the virus most was easy to spread and to be obtained in around the day to 3 sufferers was sick, and generally disappears 3 days afterwards. Whereas antibodies were just detected in around the day to 7 was sick, and the level of him increasingly increased until several days, and afterwards stabilised or descended slow up until several months. Recuperation or the death of the sufferer generally happens in around the day to 14 was sick.