Wednesday, December 12, 2012

EuroSurveillance: Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections

Eurosurveillance, Volume 17, Issue 49, 06 December 2012

We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum. 

Full Document:

  1. Institute of Virology, University of Bonn Medical Centre, Bonn, Germany
  2. These authors contributed equally to this work
  3. Ruhrlandklinik, University of Duisburg-Essen, Essen, Germany
  4. Institute of Virology, University of Duisburg-Essen, Essen, Germany
  5. Robert Koch Institute, Berlin, Germany
  6. Euroimmun AG, Lübeck, Germany
  7. TibMolbiol, Berlin, Germany
  8. Virosciences Laboratory, Erasmus MC, Rotterdam, the Netherlands

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